Glut4 antibody cell signaling
WebOct 14, 2016 · Anti-phospho-Akt (Ser-473) antibody and anti-Akt antibody were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-FLAG antibody and anti-FLAG antibody-conjugated agarose … WebGLUT4 Antibody (PA5-23052) in ICC/IF. Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase 3T3-L1 cells differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature.
Glut4 antibody cell signaling
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WebAll lanes : Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml Lane 1 : Recombinant Protein GLUT4 (Partial, Tagged) at 0.1 µg Lane 2 … WebOct 30, 2001 · The increased cell-surface GLUT4 level in the Rab5 antibody-injected cells could be caused by either a decrease in the rate of the GLUT4 internalization or a delay in GLUT4 internalization, or both. To test specifically the effect of Rab4 and Rab5 on GLUT4 internalization, 3T3-L1 adipocytes were stimulated with 1.7 nM insulin for 20 min.
Web3T3-L1 prepreadipocytes were cultured in DMEM containing 25 m m glucose, 10% fetal serum at 37 °C, and 5% CO 2.L6 muscle cells expressing c-myc epitope tagged GLUT4 (L6myc cells) were maintained in myoblast monolayer culture in α-MEM containing 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic solution (100 units/ml penicillin G, 10 μg/ml … WebAug 6, 2024 · GLUT4 and CD36 cell surface delivery is triggered by insulin- and contraction-induced signaling, which share conserved downstream effectors. ... The cells were then incubated with a combination of primary antibody pairs (anti-Rab8a (Rabbit, Cat# 55296-1-AP, Proteintech, Rosemont, IL, USA), anti-Rab11 (Rabbit, Cat# 5589S, Cell …
WebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created … WebGlut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and ...
WebOct 2, 2001 · It has been recently reported that insulin recruits a novel signaling machinery to lipid rafts required for insulin ... we verified the presence of GLUT4 by direct fluorochrome labeling of polyclonal anti-GLUT4 antibody and by using a biochemical method. Confocal microscopy analysis indicated the codistribution of GLUT4 (examined by Alexa Fluor ...
WebAll lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution Lane 1 : Human fetal heart whole cell lysate Lane 2 : … bunnings oak bench topWebOct 12, 2024 · To probe the cell signaling transduction, we determined the effect of pre-incubation with LY294002, a PI3K inhibitor, on the uptake of glucose in palmitate-treated C2C12 myotubes and the distribution of GLUT4 in plasma membrane and cytoplasm. bunnings ocean keys waWebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab216661) Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat … hall builders incWeb蛋白描述: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake By similarity. Low affinity receptor which can bind to NGF, BDNF, NT-3 ... hall builders njWebCell lysates were immunoblotted with an anti-GLUT4 antibody, anti-p-GLUT4 antibody, and anti-GAPDH antibody. (A) Panels show representative blot of GLUT4, p-GLUT4 … bunnings octopus strapshall builders shreveport louisianaWebInsulin Signaling; Warburg Effect; AMPK Signaling ... Western blot analysis of extracts of various cell lines, using GLUT4 antibody at 1:1000 dilution. Secondary antibody: HRP … hall building corp