Hieff trans
WebProduct Name: Hieff TransTM Liposomal Transfection Reagent 2. Recommended use of the chemical and restrictions on use Relevant identified uses: For research use only. Uses advised against: Not used for diagnostic procedures. 3. Details of the supplier of the safety data sheet Yeasen Biotechnology (Shanghai) Co., Ltd.
Hieff trans
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Web30 de set. de 2024 · The siRNAs (50–100 n m) were introduced into cells using Hieff Trans liposomal transfection reagent following the manufacturer’s protocol (Yeasen). Cells were harvested 48–72 h after transfection... WebProduct Description. Hieff Trans™ universal transfection reagent is a multi-purpose, convenient and efficient liposome transfection reagent developed based on the latest …
Web12 de abr. de 2024 · DMEM/F-12 with no phenol red was purchased from HyClone Proteins (Logan, UT, USA). Hieff Trans Liposomal Transfection Reagent and DH5α chemically competent cells were purchased from Yeasen Biotechnology (Shanghai, China). http://www.labbase.net/News/ShowNewsDetails-3-22-0FC567EAA023484E.html
WebHieff Trans™ PEI Transfection Reagent-GMP (Cat#40821) Description. Hieff Trans™ PEI Transfection Reagent is an ideal transfection reagent for the manufacture of AAVs, LVs, and recombinant proteins in the cell-and gene-based therapy field. It is compatible with a wide range of cell lines including the most commonly used HEK293 and CHO cells. WebHieff Trans™ universal transfection reagent is a multi-purpose, convenient and efficient liposome transfection reagent developed based on the latest nanotechnology. It is suitable for the transfection of DNA, RNA and oligonucleotides, including primary cells, Difficult-to-transfect cells, including neurons, have high transfection efficiency.
Web上海翊圣生物科技有限公司是一家集研发、生产、销售为一体的高新技术企业。翊圣生物专注于生命科学和生物技术领域，致力于为客户提供一系列高质量和有绝对价格竞争力的产品，包括分子生物学、免疫学、生物化学和细胞生物学相关的试剂、试剂盒，并提供一系列相关的技 …
Web1 de set. de 2024 · Overexpression of KLF2 in vitro inhibited growth, migration and invasion of RCC cells. Analysis of clinical specimens revealed that there is a close correlation between KLF2 and GPX4 in ccRCC. dallions meaninghttp://www.labbase.net/News/ShowNewsDetails-1-22-66A109AF7D8B18F3.html dallis goodnight collisionWeb4 de nov. de 2024 · The plasmids of pcDNA3.1-control, pcDNA3.1-NRP1(HeWu, shanghai, China), and pcDNA3.1-YTHDF1(GenePharma, Suzhou, China) were transiently transfected into cells using Hieff Trans™ Liposomal Transfection Reagent (YEASEN, Shanghai, China). Cells were harvested after transfecting 24 h to 28 h for the following experiments. dallis goodnight softballWeb1 de ago. de 2024 · Transfection with plasmid DNA was performed with Hieff trans -liposomal transfection reagent (Yeasen Biotech, China) according to the manufacturer's instructions. To obtain stably-expressing Spike protein, HEK293A cells were transiently transfected with plasmid pcDNA3.1-Spike-Myc harboring full-length SARS-CoV2 Spike … bird bedding pottery barnWebHieff TransTM complex was added to each well of the cell culture plate, followed by in-complete DMEM-H medium to a total volume of 2 mL. The culture plate was then gently shaken and mixed. After 6 h of culture at 37 °C in a 5% CO2 incubator, the growth medium was replaced. Further, after 48 h of culture, RNA was extracted, and the expression ... dallis goodnight transferWeb1 de nov. de 2024 · For a 96-well plate, 0.1 μg of DNA and 0.25 μl of Hieff Trans liposomal transfection reagent were diluted in 25 μl of Opti-MEM (Gibco). The DNA–lipid complex was added to the cells after a 20 ... dallise williams-christianWeb8 de mar. de 2024 · Lentiviruses were produced by co-transfection of the expression vector of interest with the packaging plasmids psPAX2 and pMD2G (Addgene) into HEK-293 cells using Hieff Trans™ Liposomal Transfection Reagent (Cat#40802, Yeasen, China). Virus supernatant was collected after 48 h. Transfected MM cells were screened by puromycin. bird bedding cave