Phis-sumo vector

http://www.coolaber.com/usr/2160/File/F/F4671.pdf Webb11 jan. 2016 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the above sequences into a pHis-SUMO vector (Senturia et al. …

Ligation independent cloning vectors for expression of SUMO …

Webb11 dec. 2016 · pSUMO大肠杆菌表达载体说明 WebbpET-28a (+) Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence … birthday dinner menu ideas https://lancelotsmith.com

Small Ubiquitin-Like Modifier-2 Modification of Retinoic Acid …

Webb27 feb. 2024 · However, removing SUMO is a labor- and time-consuming process. To enhance this system, here we show the construction of a bicistronic vector that allows … WebbpGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. http://www.bjbalb.com/html/Clone-Expression/YTB4021.html birthday dinner milwaukee

Champion™ pET SUMO Expression System - Thermo Fisher …

Category:高效表达可溶性重组蛋白表达载体_pHisSUMO.pdf - 豆丁网

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Phis-sumo vector

pET-N-His-PreScission-SUMO(原核表达质粒)(D2918-1μg)

WebbPhis2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions … WebbExamples of UBLs are SUMO, Rub1 (also called Nedd8), ISG15, Apg8 and Apg12. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA …

Phis-sumo vector

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Webb28 sep. 2024 · Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV … Webbsumo标签蛋白是一种小分子泛素相关修饰蛋白,是存在于真核生物中高度保守的参与蛋白质小泛素化相关修饰的一类大蛋白。 与GST、MBP或NusA相比,SUMO不仅可以作为重组 …

Webbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino … WebbpSUMO载体价格2500元,pSUMO载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为卡那,质粒大小为5598bp,是大肠杆菌蛋白表达载体。

Webb2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and … Webb28 dec. 2024 · To determine which specific SUMO form is responsible for IQGAP1 SUMOylation, three kinds of SUMO tags (pHis-SUMO1, pHis-SUMO2 and pHis-SUMO3) ...

WebbExpresso T7 SUMO Cloning Kit Container Store at -20°C Concentration Volume pETite N-His SUMO Kan Vector DNA (5 reactions) 12.5 ng/ l 15 l SUMO Positive Control C Insert …

Webbyeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, … birthday dinner mplsWebb7 juli 2014 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the ANKS3-SAM and ANKS6-SAM sequences into a pHis-SUMO … danish wire candlestick holdersWebbChampion Pet Sumo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … danish winery iowaWebbSUMO-4V constructs were made to express active recombinant proteins ending in the terminal di-glycine residues (Fig. 1, additional C-terminal residues are cleaved normally in … danish winter jacketsWebb635690: pCMV-HA Vector Set. 635689. pCMV-Myc Vector Set. Each. USD $534.00. This vector set allows for cloning and expression of your gene of interest fused to a Myc tag at either the N- or C-terminus. Notice to purchaser. Our … birthday dinner menu ideas indian non vegWebb19 sep. 2008 · A Myc-SMT3IP1 expression vector, pSG-2xMyc-SMT3IP1, was generated by inserting SMT3IP1 cDNA into pSG5-2xMyc containing two copies of a Myc-tag ... pHis … danish winter coats menWebbTumor-Linked Macrophages Promote HCC Development by Mediating the CCAT1/Let-7b/HMGA2 Signaling Pathway birthday dinner nyc group