Tacc3 oocyte

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August undefined, 2024

WebJun 28, 2024 · ( A) Immunofluorescence images of mouse oocytes fixed at different times after nuclear envelope breakdown (NEBD). Gray, microtubules (α-tubulin); green, LISD (TACC3); magenta, aMTOCs (pericentrin). Insets are magnifications of regions outlined by … WebMar 21, 2024 · TACC3 (Transforming Acidic Coiled-Coil Containing Protein 3) is a Protein Coding gene. Diseases associated with TACC3 include Gliosarcoma and Giant Cell Glioblastoma. Among its related pathways are Regulation of activated PAK-2p34 by …

Proteomic profiling of murine oocyte maturation - Vitale - 2007 ...

WebTransforming acidic coiled-coil-containing protein 3 is a protein that in humans is encoded by the TACC3 gene. [5] [6] The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. WebJul 15, 2024 · We speculated that the function of TPX2 beyond microtubule assembly is conserved among species and may also be related to AURKA and TACC3. Here, porcine oocytes were selected to reveal the spatiotemporal and function of TPX2 in microtubule … ninja chopper food processor

TACC3 Gene - GeneCards TACC3 Protein TACC3 Antibody

WebAug 5, 2024 · TACC3, a member of the TACC family, is a dominant protein in this domain, which is essential to promote the formation of a highly dynamic liquid-like meiotic spindle domain (LISD) in mammalian oocytes (Peset and Vernos 2008; Wang et al. 2024). WebMay 1, 2024 · During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified. ... SLBP (Abnova H00007884-M01, 200 ng/mL), tubulin (Sigma T8203, 10 μg/mL) and TACC3 ... WebJul 13, 2015 · As a result of the embryonic lethality, the function of TACC3 during oocyte meiosis remains unclear. Compared to mitosis, little is known about the mechanisms that drive and control meiosis in oocytes. Here, we show that TACC3 is expressed around the … ninja christmas coloring page

Cell‐specific network analysis of human folliculogenesis reveals ...

The role of TACC3 in mitotic spindle organization - Ding

WebAug 5, 2024 · Notably, IPZ treatment decreased TPX2 expression and abnormal subcellular localization. Furthermore, the expression levels of aurora kinase A (AURKA) and transforming acidic coiled-coil 3 (TACC3)... WebFeb 25, 2024 · Oocyte treatments with taxol, nocodazole, or cold did not affect CLTC expression levels but led to disorders of its distribution. Functional impairment of CLTC by specific morpholino injections in GV-stage oocytes led to disruptions in spindle assembly and chromosomal alignment, accompanied by impaired first polar body (PB1) emissions. nug36200 eatonWebMay 1, 2002 · D-TACC is also requiredto efficiently recruit Msps to the poles of the acentrosomal meiotic spindles in oocytes, suggesting that these proteins can work together to regulate microtubule behaviour even when centrosomes are not present [25. ... Thus, perhaps the TACC3 −/ ... ninja christmas tree ornament

"WebApr 26, 2024 · In summary, we conclude that AURKA is the only Aurora kinase in mouse oocytes that is essential for fertility and MI [ 30, 31] ( Fig 8 ). Its unique functions include, at least, initiating MTOC fragmentation through activation of PLK1 and spindle formation … " - Tacc3 oocyte

Tacc3 oocyte

National Center for Biotechnology Information

WebTACC3; transforming acidic coiled-coil containing protein 3. Aliases: ERIC1, Tacc4, ERIC-1, maskin. Location: 4p16.3. Summary: This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may … WebJul 13, 2015 · TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to …

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WebSep 19, 2005 · TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. … WebJul 13, 2015 · TACC3 is expressed in oocytes and in early cleavage stage pre-implantation embryos. The expression of TACC3 mRNA in bovine oocytes and in vitro produced pre-implantation embryos was examined using qRT-PCR. TACC3 expression was detected at similar levels (p>0.05) throughout oocyte maturation and during the first cleavage stages …

WebMar 1, 2024 · Cobalt chloride disrupted spindle formation by affecting TACC3 and decreasing Ac-a-tubulin levels, altered oocyte actin distribution and polarity, and impaired the mitochondrial structure and functions. The results provide in vivo evidence for cobalt chloride negatively affecting oocyte development. 2. Materials and methods 2.1. Chemicals WebNov 1, 2002 · A protein spot cored from a silver-stained two dimensional (2D) gel of germinal vesicle stage immature mouse oocytes was identified as Transforming Acidic Coiled Coil containing protein (TACC3)...

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WebJun 28, 2024 · Abstract. Mammalian oocytes segregate chromosomes with a microtubule spindle that lacks centrosomes, but the mechanisms by which acentrosomal spindles are organized and function are largely unclear. In this study, we identify a conserved …

WebTransforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes... nufs kitchen cleethorpesWebJun 2, 2024 · TACC3, a transforming acidic coiled-coil (TACC) family member, is frequently upregulated in a broad spectrum of cancers, including breast cancer. It plays critical roles in protecting microtubule stability and centrosome integrity that is often dysregulated in cancers; therefore, making TACC3 a highly attractive therapeutic target. nufs office365WebNational Center for Biotechnology Information nuft softwareWebMar 1, 2024 · Spindle assembly defects mediated by centrin TACC3 and decreased acetylation, microﬁlament reorganization failure, and mitochondrial dysfunction were found to be the underlying mechanisms involved in the toxicity of cobalt chloride to oocytes. nug5075bfb2 specsWebNov 18, 2024 · We found that mutations in TACC3 cause defects in spindle assembly by disrupting the structure of the huoMTOC, which leads to clinical oocyte maturation arrest. This suggests that the huoMTOC might be an important biomarker for evaluating the … nuf vs asWebOct 14, 2024 · The primary antibodies were listed as follows: MTX2 (1:100 in dilution, 11610-1-AP, Proteintech), GDF9 (1:100 in dilution, ab93892, Abcam), TACC3 (1:50 in dilution, ab134154, Abcam), EIF5A (1:50 in dilution, 11309-1-AP, Proteintech), ASPM (1:150 in dilution, 26223-1-AP, Proteintech). ninja city cleveland downtownWebOct 16, 2006 · Proteins from 500 germinal vesicle (GV) and metaphase II- (MII) arrested oocytes were extracted, resolved on 2D electrophoretic gels, and stained with silver. Analysis of the gels indicated that 12 proteins appeared to … ninja city cleveland